Detection of Tospoviruses in Ornamental Plants in Hobby Gardens and Landscaping Areas in Adalar District of Istanbul Province

Authors

  • Fatma Şafak İstanbul İl Gıda Tarım ve Hayvancılık Müdürlüğü, 34728 İstanbul
  • Muharrem Arap Kamberoğlu Çukurova Üniversitesi

DOI:

https://doi.org/10.24925/turjaf.v5i9.1119-1124.1404

Keywords:

Ornamentals, TSWV, INSV, IYSV, RT-PCR, ELISA

Abstract

This study was conducted in order to detect Tomato spotted wilt virus (TSWV), Irish yellow spot virus (IYSV) and Impatients necrotic spot virus (INSV) in ornamental plants growing in hobby gardens and landscaping areas in Adalar district (Büyükada, Heybeliada, Kınalıada and Burgazada) of Istanbul province between 2015 and 2016. During the surveys carried out in that district, the samples were collected from both simptomatologically suspicious ornamental plants and the plants which did not show any symptoms. All of the collected samples (n=150) were firstly tested by Double Antibody Sandwich (DAS) ELISA and none of the samples were found to be infected with TSWV and INSV. The samples detected to be positive with IYSV by ELISA tests were then used in RT-PCR studies. At the RT-PCR using the IYSV-465c; IYSV-239f primer pair, a band with a size of 240 bp was observed for Pittosporum tobira and Hydrangea macrophylla. Therefore, the presence of IYSV infection in Adalar was also confirmed molecularly.

Author Biography

Muharrem Arap Kamberoğlu, Çukurova Üniversitesi

Çukurova Üniversitesi Ziraat Fakültesi Bitki Koruma Bölümü, Yardımcı Doçent

Published

12.09.2017

How to Cite

Şafak, F., & Kamberoğlu, M. A. (2017). Detection of Tospoviruses in Ornamental Plants in Hobby Gardens and Landscaping Areas in Adalar District of Istanbul Province. Turkish Journal of Agriculture - Food Science and Technology, 5(9), 1119–1124. https://doi.org/10.24925/turjaf.v5i9.1119-1124.1404

Issue

Section

Crop Production