Determination and Comparison of In Vitro Radical Scavenging Activity of Both Garlic Oil and Aqueous Garlic Extracts and Their In Vivo Antioxidant Effect on Schistosomiasis Disease in Mice

Authors

  • Somayye Sadrefozalayi Department of Biology, Faculty of science, Urmia University, Urmia, I.R.
  • Behnaz Aslanipour Department of Bioengineering, Faculty of Engineering, Ege University, 35100 Bornava, Izmir
  • Murat Alan Department of Obstetrics and Gynecology, Izmir Tepecik Training and Research Hospital, 35120 Tepecik, Izmir,
  • Mehmet Calan Department of Internal Medicine, Izmir Bozyaka Training and Research 14 Hospital, 35170 Bozyaka, Izmir

DOI:

https://doi.org/10.24925/turjaf.v6i7.820-827.1647

Keywords:

Garlic extracts, Antioxidant, Radical scavenging activity, schistosomiasis, worms

Abstract

Garlic has long been utilizing as a folk remedy for various diseases. In this study, the effect of aqueous garlic extract (AGE) (125 mg kg-1), garlic oil extract (GOE) (125 mg kg-1), and the combination of both extracts (AGE+GOE) (62.5 from each) was studied over a parasitic disease namely schistosomiasis. Determination of some radical scavenging activities including Ferric Reducing Ability Power, Hydrogen peroxide, DPPH and nitric oxide radical scavenging activities were studied. The antioxidant properties of the mentioned extracts on both normal and Schistosoma mansoni-infected mice was also studied. The liver tissues were analyzed for the level of Malondialdehyde (MDA) and glutathione (GSH) known as biomarkers for oxidative and reducing status, respectively. The results showed that almost all tested extracts showed high effect of radical scavenging activity. Furthermore, remarkable decrease in worms and tissue eggs were analyzed in all tested extracts as AGE+ GOE (1015 ± 1.41) represented higher level of prohibition in total number of worms and eggs compared to infected untreated mice (1502 ± 2.13). The garlic extracts were potent antioxidants with slight differences.

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Published

16.07.2018

How to Cite

Sadrefozalayi, S., Aslanipour, B., Alan, M., & Calan, M. (2018). Determination and Comparison of In Vitro Radical Scavenging Activity of Both Garlic Oil and Aqueous Garlic Extracts and Their In Vivo Antioxidant Effect on Schistosomiasis Disease in Mice. Turkish Journal of Agriculture - Food Science and Technology, 6(7), 820–827. https://doi.org/10.24925/turjaf.v6i7.820-827.1647

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Section

Research Paper