The Assessment of Androgenic Response of Two Nematode Resistant Pepper (Capsicum annuum L.) Genotypes

Authors

DOI:

https://doi.org/10.24925/turjaf.v7i12.2103-2110.2828

Keywords:

Anther culture, Genotype, Haploid plant production, Nematode resistance, Cold pre-treatment

Abstract

Pepper is one of the most cultivated vegetables worldwide and also consumed substantially as a flavouring ingredient in different culinary traditions. Therefore, many researchers have focused on its breeding to develop new varieties. One of plant breeding aims is to attain disease and pest resistance. The use of tissue culture methods in plant breeding has many advantages. The response of two nematode resistant pepper genotypes to the anther culture and the effect of cold pre-treatment to the floral buds have been investigated in this study. Alata 2095 and Alata 2096 both specified as resistant to the nematode by Alata Horticultural Research Institute (Alata, Mersin, Turkey) were used as plant material. Two pre-treatments were used in this study: cold and no cold. In cold treatment, flower buds were kept in fridge at 4°C for 24 hours prior to the anther culture. Murashige and Skoog medium contained 0.25% activated charcoal, 6.5 g L-1 agar, 0.5 mg L-1 6-benzyl-amino-purine (BAP), 4 mg L-1 naphthalene-acetic-acid (NAA), 15 mg L-1 silver nitrate (AgNO3), and 30 g L-1 sucrose was used. After assessment, the highest mean of plant number was 39.08 per 100 anthers for Alata 2095 genotype. It was 46.61 and 31.56 in cold and no cold treatment, respectively. For Alata 2096 genotype, the mean was 1.96 per 100 anthers (1.68 and 2.25 in cold and no cold treatment, respectively). Statistical analyses confirmed that there was significant difference between treatments, genotypes and also significant interaction between those factors. At the end of the study, we can say that Alata 2095 genotype has a good androgenic response and it can be beneficial in further pepper breeding studies.

Author Biography

Davut Keleş, Alata Horticultural Research Institute, Erdemli-Mersin

Dr.

Downloads

Published

14.12.2019

How to Cite

Shimira, F., Keleş, D., Taşkın, H., & Abak, K. (2019). The Assessment of Androgenic Response of Two Nematode Resistant Pepper (Capsicum annuum L.) Genotypes. Turkish Journal of Agriculture - Food Science and Technology, 7(12), 2103–2110. https://doi.org/10.24925/turjaf.v7i12.2103-2110.2828

Issue

Section

Research Paper

Most read articles by the same author(s)