Optimization of Plant Regeneration in Different Pepper (Capsicum annuum L.) Lines

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DOI:

https://doi.org/10.24925/turjaf.v8i2.471-477.3207

Keywords:

Tissue culture, organogenesis, plant growth regulators, shoot formation, Callus

Abstract

Development of an efficient plant regeneration protocol is essential for vegetable crop advancement by biotechnological methods. In this study, regeneration protocols of four pepper lines of different pepper types were optimized. Different protocols for organogenesis were investigated in regeneration experiments. Optimum plant regeneration was obtained in different combinations of 6-Benzylaminopurine (BAP) and Indole-3-acetic acid (IAA) in organogenesis assays. In organogenesis experiment, the highest shoot development was determined as 80% from hypocotyl explant of Demre pepper in 4 mg L-1 BA+0.5 mg L-1 IAA, 80% from hypocotyl explant of Charleston pepper in 3 mg L-1 BA+0.5 mg L-1 IAA, 80% from hypocotyl explant of capia pepper in 5 mg L-1 BA+0.5 mg L-1 and 84% from hypocotyl explant of bell pepper in 5 mg L-1 BA+0.5 mg L-1 IAA. Afterward, shoots were rooted and whole plants were obtained.

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Published

28.02.2020

How to Cite

İzgü, T., İlbi, H., & Mendi, Y. Y. (2020). Optimization of Plant Regeneration in Different Pepper (Capsicum annuum L.) Lines. Turkish Journal of Agriculture - Food Science and Technology, 8(2), 471–477. https://doi.org/10.24925/turjaf.v8i2.471-477.3207

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Section

Research Paper