In Vitro Direct Organogenesis Using Mature Embryo With Cotyledons In Chickpea

Authors

  • Ali Ammar Regional Agricultural Research Institute, 60800, Bahawalpur
  • Mahmood Ayyaz Department of Plant production and Technologies, Faculty of Agricultural Sciences and Technologies, Niğde University, Turkey
  • Ahsan Irshad National Centre of Space Mutagensis for Crop Improvement, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, 100081, Beijing
  • Syeda Farhana Bukhari Department of Plant Breeding and Genetics, University College of Agriculture, Bahauddin Zakariya University, 60800, Multan,
  • Ghulam Yasin Institute of Pure and Applied Biology, Bahauddin Zakariya University, Multan, 60800
  • Seema Mahmood Mahmood Institute of Pure and Applied Biology, Bahauddin Zakariya University, Multan, 60800
  • Shoaib Ur Rehman Key laboratory for crop germplasm resources and utilization, the national key facility for crop gene resources and genetic improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, 100081, Beijing

DOI:

https://doi.org/10.24925/turjaf.v4i1.17-21.471

Keywords:

BAP, Chickpea, Somatic Embryogenesis, IAA, multiple shoot regeneration, MS, medium

Abstract

The present study was conducted to find out an in vitro efficient method for multiple shoot regeneration of two local chickpea varieties. The mature embryos were excised of two chickpea varieties i.e. Bittle-98 and Dasht-2000 (with cotyledon and without cotyledon) used as explants. The explants were cultured on Murashige and Skoog (MS) medium supplemented with three concentrations of (2, 3, 4 mg/l) 6-benzylaminopurine (BAP) using explant with and without cotyledon. Further, 0.5 mg/l α-naphthaleneacetic acid (NAA) along with varied concentrations of BAP (2, 3, 4 mg/ l) was also tested using explant with cotyledon. 3 mg/l BAP alone and 3 mg/l BAP with 0.5 mg/l NAA were found the most effective cytokinin in multiple shoot induction in both tested varieties. Bittle-98 and Dasht-2000 showed 82% and 76% elongation in shoots induction with 0.2 mg/l Indole-3-acetic acid (IAA). Root formation was recorded 80% and 60% in Bittle-98 and Dasht-2000 with 1.0 mg/l Indole-3-butyric acid (IBA). Whereas, recorded root formation was 40 and 20% in Bittle-98 and Dasht-2000 with 1.0 mg/l NAA. The best response for rooting observed in Bittle-98 as its roots were thick, long and strong. Plantlets of Bittle-98 were acclimatized in solid medium for 7-14 days. The successful invitro regeneration of Bittle-98 was observed, when excised embryo with cotyledon were used as explant, made it valuable for genetic transformation.

Author Biography

Mahmood Ayyaz, Department of Plant production and Technologies, Faculty of Agricultural Sciences and Technologies, Niğde University, Turkey

PhD student at Department of Plant production and Technologies, Faculty of Agricultural Sciences and Technologies, Niğde University, Turkey

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Published

19.01.2016

How to Cite

Ammar, A., Ayyaz, M., Irshad, A., Bukhari, S. F., Yasin, G., Mahmood, S. M., & Rehman, S. U. (2016). In Vitro Direct Organogenesis Using Mature Embryo With Cotyledons In Chickpea. Turkish Journal of Agriculture - Food Science and Technology, 4(1), 17–21. https://doi.org/10.24925/turjaf.v4i1.17-21.471

Issue

Section

Agricultural Technologies