Molecular Investigation and Phylogenetic Analysis of Ehrlichia canis in Dogs in Siirt, Turkey

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DOI:

https://doi.org/10.24925/turjaf.v10i10.1832-1837.5338

Keywords:

Ehrlichia canis, Phylogeny, PCR, Siirt, Turkey

Abstract

Ehrlichia canis is the primary etiologic agent of canine monocytic ehrlichiosis, a tick-transmitted disease of dogs. The aim of this study is to molecularly investigate the presence of E. canis and to reveal its prevalence in dogs in Siirt province. The animal material of the study is consisted of a total of 82 dogs. A region of the 16S ribosomal RNA gene of E. canis was targeted for PCR amplification. As a result of the conducted Nested-PCR, positivity was detected at the rate of 10.53% (4/38) in male dogs and 13.64% (6/44) in females, and Ehrlichia canis specific bands of size 389 bp were obtained in 10 (12.20%) dogs in total. The phylogenetic tree was constructed with the Maximum Likelihood (MCL) method, The nucleotide sequence was registered in the NCBI GenBank database with access numbers OK331365.1-OK331366. Early detection of the disease by means of hematological, serological, or molecular tests is very important in terms of prognosis. More studies should be performed to determine vector-disease relationships in this region about ticks that vector the disease.

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Published

12.10.2022

How to Cite

Aslan Çelik, B., Çelik, Özgür Y., Yılmaz, A. B., Ayan, A., Orunç Kılınç, Özlem, Özdemir, R., & Oktay Ayan, Özge. (2022). Molecular Investigation and Phylogenetic Analysis of Ehrlichia canis in Dogs in Siirt, Turkey. Turkish Journal of Agriculture - Food Science and Technology, 10(10), 1832–1837. https://doi.org/10.24925/turjaf.v10i10.1832-1837.5338

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Section

Research Paper

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