Determination of Toll-Like Receptor 1 Gene Polymorphisms in Zavot, Turkish Grey, East Anatolian Red, Anatolian Black and South Anatolian Red Cattle Breeds

Authors

  • Mehmet Ulaş Çınar Department of Animal Science, Unit of Biometry and Genetics, Faculty of Agriculture, Erciyes University, 38039 Kayseri
  • Korhan Arslan Genetics Department, Faculty of Veterinary Medicine, Erciyes University, 38039 Kayseri
  • Esma Gamze Ilgar Genetics Department, Faculty of Veterinary Medicine, Erciyes University, 38039 Kayseri
  • Bilal Akyüz Genetics Department, Faculty of Veterinary Medicine, Erciyes University, 38039 Kayseri

DOI:

https://doi.org/10.24925/turjaf.v4i5.352-355.594

Keywords:

TLR1 gene, Turkish cattle breeds, Allele frequency, Genotype frequency, Genetic polymorphism

Abstract

Toll-like receptors (TLRs) play an important role in non-specific immunity against different infectious agents such as bacterium or parasite. The aim of this work was to investigate the allele and genotype frequencies of three single-nucleotide polymorphisms (SNPs) in bovine TLR1 gene in native Turkish cattle breeds. DNA samples were extracted using the phenol chloroform protocol from 77 Zavot, 60 Turkish Grey, 51 East Anatolian Red, 69 Anatolian Black and 46 South Anatolian Red cattle. Target regions of the TLR1 gene were digested BsII and HpyI88III restriction enzymes. Results showed that the (A) allele frequency had higher in all native Turkish cattle breeds of the TLR1-G1409A locus. The (F) allele frequency was found to be higher compared to (E) allele in the TLR1-G1550A site. The frequencies of both (C) and (T) alleles were close to each other in the TLR1-C632T site. In conclusion genetic polymorphisms exist in Turkish native cattle populations in terms of known TLR1 variants.

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Published

12.05.2016

How to Cite

Çınar, M. U., Arslan, K., Ilgar, E. G., & Akyüz, B. (2016). Determination of Toll-Like Receptor 1 Gene Polymorphisms in Zavot, Turkish Grey, East Anatolian Red, Anatolian Black and South Anatolian Red Cattle Breeds. Turkish Journal of Agriculture - Food Science and Technology, 4(5), 352–355. https://doi.org/10.24925/turjaf.v4i5.352-355.594

Issue

Section

Animal Production